New DNA Detection Technology and its Application to Genetic Analysis of Rice: Could Lead to Developing New Cultivars

March 06, 2023

A joint research group of Professor YAMASAKI Masanori of the Faculty of Agriculture, Niigata University, Rym Fekih, an academic researcher at Kobe University, and OBANA Takahiro, Marketing Manager of Eurofins Genomics has succeeded the detailed DNA analysis in Japanese rice, which was previously considered difficult to study. The new technology called GRAS-Di (Genotyping by Random Amplicon Sequencing-Direct) developed by Toyota Motor Corporation is highly reproducible, accurate, and easy to implement at a low cost. This technology will innovate the genetic analysis of Japanese rice and can be applied to other plants as well as to animals and microorganisms. It is also expected to promote the development of new cultivars using the experimental large-scale Japanese rice population currently being cultivated by Niigata University.

Research Results

  • GRAS-Di technology is effective for genotyping of Japanese rice with high reproducibility and accuracy, can be performed easily at low cost, and is expected to be applied to other organisms.
  • GRAS-Di technology has accelerated the analysis of Japanese rice and enabled detailed genetic analysis.

Publication Details

Journal: Plants
Title: High-Density Linkage Maps from Japanese Rice japonica Recombinant Inbred Lines Using Genotyping by Random Amplicon Sequencing-Direct (GRAS-Di)
Authors: Rym Fekih, Yohei Ishimaru, Satoshi Okada, Michihiro Maeda, Ryutaro Miyagi, Takahiro Obana, Kazuyo Suzuki, Minoru Inamori, Hiroyuki Enoki and Masanori Yamasaki
doi: 10.3390/plants12040929

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